Journal: Gels

Article Title: Whey Protein Isolate Hydrogels Containing Cannabidiol Support the Proliferation of Pre-Osteoblasts

doi: 10.3390/gels11060418

Figure Lengend Snippet: The results of % cell viability and proliferation of MC3T3-E1 pre-osteoblasts seeded on WPI/CBD hydrogels relative to WPI0 (set as 100%). TCPS is tissue-culture-treated polystyrene and serves as control. Absorbance readings at 570/600 nm were taken at days 3 and 7. Each bar represents the mean ± SD of n = 6 (** p < 0.01, **** p < 0.0001; compared to the WPI control) ( ## p < 0.01, #### p < 0.0001; compared to the TCPS control).

Article Snippet: Cellular viability and behaviour assays were conducted utilising the mouse calvaria osteoblast precursor cell line (MC3T3-E1, DSMZ Braunschweig, Germany, ACC-210).

Techniques: Control

Journal: Gels

Article Title: Whey Protein Isolate Hydrogels Containing Cannabidiol Support the Proliferation of Pre-Osteoblasts

doi: 10.3390/gels11060418

Figure Lengend Snippet: SEM images demonstrating the cellular morphology of MC3T3-E1 pre-osteoblasts on WPI/CBD hydrogels. Images were taken at 7 and 10 days of culture ( middle and lower panels ). The upper panel shows unseeded hydrogels (without cells) to facilitate comparison. Scale bars represent 10 μm.

Article Snippet: Cellular viability and behaviour assays were conducted utilising the mouse calvaria osteoblast precursor cell line (MC3T3-E1, DSMZ Braunschweig, Germany, ACC-210).

Techniques: Comparison

Journal: Gels

Article Title: Whey Protein Isolate Hydrogels Containing Cannabidiol Support the Proliferation of Pre-Osteoblasts

doi: 10.3390/gels11060418

Figure Lengend Snippet: Assessment of the osteogenic potential of MC3T3-E1 pre-osteoblastic cells cultured on WPI/CBD hydrogels; ( a ) the expression of normalised ALP-specific activity on days 3, 7, and 14, ( b ) collagen production, and ( c ) calcium production by pre-osteoblasts on days 7, 14, and 21. Each bar represents the mean ± SD of n = 6 (* p < 0.05, ** p < 0.01, **** p < 0.0001; compared to the WPI0 control) ( # p < 0.05, ## p < 0.01, ### p < 0.001, #### p < 0.0001; compared to the TCPS control), while the absence of asterisks in the graph of calcium production ( c ) signifies statistically non-significant differences when compared to the WPI0 control.

Article Snippet: Cellular viability and behaviour assays were conducted utilising the mouse calvaria osteoblast precursor cell line (MC3T3-E1, DSMZ Braunschweig, Germany, ACC-210).

Techniques: Cell Culture, Expressing, Activity Assay, Control